|
| Status |
Public on Sep 01, 2011 |
| Title |
Adipose tissue 43 |
| Sample type |
RNA |
| |
|
| Source name |
Subcutaneous adipose tissue
|
| Organism |
Homo sapiens |
| Characteristics |
individual: volunteer 18
disease state: metabolic syndrome
batch: 1
n-3 pufa intake g/day: 2.144
gender: M
tissue: subcutaneous adipose tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen RNeasy extraction kits
|
| Label |
biotin
|
| Label protocol |
2 μg of high quality total RNA was used with the Affymetrix Eukaryotic One-Cycle Target Labeling and Control reagents to generate Biotin-labeled antisense cRNA
|
| |
|
| Hybridization protocol |
The labeled and fragmented cRNA was hybridized to Affymetrix nugohs1a520180 Genechips for 16 hr at 45C. Genechips were washed and stained using the Affymetrix fluidics Station 400.
|
| Scan protocol |
Affymetrix arrays were scanned using the Affymetrix 3000G scanner.
|
| Description |
100678-43.CEL
Subcutaneous adipose tissue sample from volunteer 18
|
| Data processing |
The data were quality checked and analyzed using the R programming language. The GCRMA algorithm was used for background correction and normalization.
|
| |
|
| Submission date |
Mar 21, 2011 |
| Last update date |
Sep 01, 2011 |
| Contact name |
Helen M Roche |
| E-mail(s) |
[email protected]
|
| Organization name |
University College Dublin
|
| Lab |
Nutrigenomics Research Group
|
| Street address |
UCD Conway Institute of Biomolecular and Biomedical Research
|
| City |
Dublin |
| State/province |
Belfield |
| ZIP/Postal code |
Dublin 4 |
| Country |
Ireland |
| |
|
| Platform ID |
GPL7020 |
| Series (1) |
| GSE28070 |
Transcriptomic response to variation in habitual n-3 polyunsaturated fatty acid intake |
|
