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Sample GSM694371 Query DataSets for GSM694371
Status Public on Jun 01, 2011
Title NSC-2
Sample type RNA
 
Source name mouse NSC
Organism Mus musculus
Characteristics cell type: neural stem cells
genotype: p53-/-
strain: BL6
tissue: brain
age: E14.5 embryo
Growth protocol NSCs were prepared from embryonic day 14.5 mouse telencephalon and expanded in serum-free Dulbecco's Modified Eagle's medium (DMEM) /F12 medium containing bovine insulin (10 μg/ml), human transferrin (100 μg/ml), BSA (100 μg/ml), progesterone (60 ng/ml), putrescine (16 μg/ml), sodium selenite (40 ng/ml), N-acetylcysteine (60 μg/ml), forskolin (5 μM), PDGF (10 ng/ml), penicillin, streptomycin, and bFGF as floating spheres. Astrocytes were induced in NSC cultures by changing the medium to DMEM with 10% FCS for 3 wks. OPCs were induced by culturing NSCs in DMEM containing PDGFAA (10 ng/ml), bFGF (2 ng/ml), and 0.25% FCS and purified by sequential immunopanning.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared using a RNeasy Mini Kit (QIAGEN).
Label biotin
Label protocol Five micrograms of total RNA were labeled with biotin by in vitro transcription (IVT) using the One-Cycle Target Labeling procedure for biotin labeling by in vitro transcription (Affymetrix), according to the manufacturer’s instruction.
 
Hybridization protocol Labeled probes were used for the GeneChip Mouse Genome 430 2.0 array (Affymetrix) according to the manufacturer’s instructions.
Scan protocol The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix) to generate CEL data.
Data processing The CEL data were then analyzed with dChip software, which can normalize and process multiple datasets simultaneously.
 
Submission date Mar 22, 2011
Last update date Jun 01, 2011
Contact name Toru Kondo
E-mail(s) [email protected]
Phone +81-11-706-6082
Organization name Hokkaido University
Department Institute for Genetic Medicine
Lab Division of Stem Cell Biology
Street address Kita-15, Nishi-7, Kita-ku,
City Sapporo
ZIP/Postal code 060-0815
Country Japan
 
Platform ID GPL1261
Series (1)
GSE28091 Expression profiles of mouse glioma-initiating cells.

Data table header descriptions
ID_REF
VALUE log2 RMA processed signal

Data table
ID_REF VALUE
1415670_at 10.17944712
1415671_at 10.55804269
1415672_at 10.87304356
1415673_at 10.42880228
1415674_a_at 9.973168171
1415675_at 9.494759524
1415676_a_at 11.24404259
1415677_at 8.673197383
1415678_at 9.552552757
1415679_at 10.7323301
1415680_at 9.952604762
1415681_at 9.644772647
1415682_at 7.855736897
1415683_at 11.31213846
1415684_at 7.934316617
1415685_at 8.398535476
1415686_at 9.121516245
1415687_a_at 12.13020063
1415688_at 10.07861819
1415689_s_at 8.172565586

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM694371.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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