|
| Status |
Public on Mar 25, 2011 |
| Title |
SL2 cells treated with Tis11 dsRNA and LPS, biological rep. 1 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila melanogaster SL2 cells; Tis11-dsRNA; LPS
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: SL2
treatment: Tis11 dsRNA depletion and LPS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using RNEasy kit from Qiagen (Cat. No. 74106) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Labeling was performed according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev. 2), using biotin as label.
|
| |
|
| Hybridization protocol |
Fragmentation of cRNA and subsequent hybridization (16 hours) was performed according to Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev. 2), using a Hyb Oven 640 and a Fluidics Station FS450.
|
| Scan protocol |
GeneChips were scanned using the Scanner 3000 7G.
|
| Data processing |
The RMA algorithm (Robust Multichip Average) was used for normalization of raw data (RMAExpress software, http://rmaexpress.bmbolstad.com/).
|
| |
|
| Submission date |
Mar 24, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Milan Spasic |
| Organization name |
German Cancer Research Centre
|
| Street address |
Im Neuenheimer Feld 280
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE28147 |
Analysis of gene expression regulated by Drosophila melanogaster Tis11 |
|
| Relations |
| Reanalyzed by |
GSE119084 |
