|
| Status |
Public on Mar 26, 2011 |
| Title |
Heart_double_knockout_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
E9.25 knockout mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype/variation: IP3R1−/−IP3R3−/−
|
| Treatment protocol |
Animals were housed under a 12 h light/12 h dark cycle and ad-lib access to food and water. Animals were sacrificed by cervical dislocation.
|
| Growth protocol |
Embryos were grown until embryonic day 9.25.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction using RNeasy (QIAgen) was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Fragmented and biotinylated cDNAs were prepared from the total RNA according to NuGEN protocol (FL-Ovation cDNA Biotin Module V2 USER GUIDE Version 04.05.07).
|
| |
|
| Hybridization protocol |
cDNAs were hybridized on GeneChip arrays and the arrays were washed and stained in Affymetrix Fluidics Station 450 according to the standard Affymetrix protocol (Expression Analysis Technical Manual P/N 702232 Rev.2).
|
| Scan protocol |
GeneChip arrays were scanned using Affymetrix GeneChip 3000 7G scanner. GeneChip arrays were scanned using Affymetrix GeneChip 3000 7G scanner.
|
| Description |
4(12-7-92-5).CEL
|
| Data processing |
RMA, Signal calculated by GCOS1.4 software
|
| |
|
| Submission date |
Mar 25, 2011 |
| Last update date |
Mar 26, 2011 |
| Contact name |
Keiko Uchida |
| E-mail(s) |
[email protected]
|
| Organization name |
Keio University School of Medicine
|
| Street address |
35 Shinanomachi
|
| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE28186 |
Expression data from from the E9.25 hearts of mouse embryos |
|
