|
| Status |
Public on Apr 02, 2011 |
| Title |
Control_2 |
| Sample type |
RNA |
| |
|
| Source name |
Control
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: healthy (non EBS)
cell type: Keratinocytes
tissue origin: skin biopsies with no stimulation
|
| Treatment protocol |
No treatment before extraction
|
| Growth protocol |
The Biopsies were mechanically homogenized (Rotor/Stator homogeniser, PowerGen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNEasy Fibrous Tissue Midi Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Probe labelling was done at the McGill University and Genome Quebec Innovation Centre (www.genomequebec.mcgill.ca) and was performed in parallel for control and EBS samples.
|
| |
|
| Hybridization protocol |
Hybridation was done at the McGill University and Genome Quebec Innovation Centre (www.genomequebec.mcgill.ca) and was performed in parallele for control and EBS samples.
|
| Scan protocol |
Scanning was done using a GeneArray Scanner at the McGill University and Genome Quebec Innovation Centre (www.genomequebec.mcgill.ca) and was performed in parallel for control and EBS samples
|
| Description |
Gene expression data from non EBS patients subject, without any stimulation.
|
| Data processing |
The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix) and the raw image fils obtained (.CEL) were used for statistical analyses. Affy and Limma packages in Bioconductor (www.bioconductor.org) using R2.12.0 were used to perform quality tests, RMA normalization and Smyth's moderated t-test.
|
| |
|
| Submission date |
Apr 01, 2011 |
| Last update date |
Apr 02, 2011 |
| Contact name |
Catherine Laprise |
| Organization name |
Université du Québec à Chicoutimi
|
| Street address |
555 Boul. Université
|
| City |
Chicoutimi |
| State/province |
Qc |
| ZIP/Postal code |
G7H 2B1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE28315 |
Gene expression pattern of skin biopsies of epidermolysis bullosa simplex patients in comparison with control subjects |
|
