|
| Status |
Public on Apr 03, 2011 |
| Title |
ES_Day5_2 |
| Sample type |
RNA |
| |
|
| Source name |
ES cells differentiated on OP9 stromal cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Day5 control
cell line: Murine ES cells E14tg2a
|
| Treatment protocol |
At Day 5 Notch OFF cells were cultured in media with TET and Notch ON cells were cultured in media without TET
|
| Growth protocol |
Murine ES cells were grown on a confluent OP-9 cells and differentiate along the hematopoitc lineage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted on Day 5 and Day 8 using Qiagen microRNeasy kit
|
| Label |
Hy5
|
| Label protocol |
Labeled according to the manufactures protocol
|
| |
|
| Hybridization protocol |
Hyb was performed with Agilent hybridization SureHyb chamber kit and gasket slide kit according to manufacture
|
| Scan protocol |
Scanning was done on a GenePix 4100A scanner using GenePix 6.0 pro software at 10 micro and PMT 750
|
| Description |
Control
|
| Data processing |
Averages were calculated for replicate probes and probles were excluded if they had average value below a cutoff value of 100 or less than a value for the negative control (median value of negative control plus 3SD). Data was normalized by calculating the media value for the the probes with the lowest CV and then using it as normalization factor across samples. Data was processed on GenePix 6.0 and analyzed in Microsoft Excel.
|
| |
|
| Submission date |
Apr 02, 2011 |
| Last update date |
Apr 03, 2011 |
| Contact name |
Habib Hamidi |
| E-mail(s) |
[email protected]
|
| Phone |
5105892147
|
| Organization name |
UCLA
|
| Street address |
2825 Santa Monica Blvd., Suite 200
|
| City |
Santa Monica |
| State/province |
CA |
| ZIP/Postal code |
90404 |
| Country |
USA |
| |
|
| Platform ID |
GPL11432 |
| Series (1) |
| GSE28338 |
MicroRNA expression during Hematopoiesis |
|
