|
| Status |
Public on Jan 22, 2024 |
| Title |
Mitochondrial transfer 1 |
| Sample type |
RNA |
| |
|
| Source name |
NK-92MI cell after mitochondrial transfer
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: NK-92MI cell
|
| Treatment protocol |
Transfer of mitochondria isolated from WRL-cells (5 ug) into NK-92MI cells by centrifugation at 1,500 × g for 15 min
|
| Growth protocol |
NK-92MI cells were cultured in a-MEM medium supplemented with 12.5% FBS, 12.5% horse serum, 0.1 mM 2-mercaptpethanol, 0.2 mM myo-inositol, 0.02 mM folic acid
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from NK-92MI cells was isolated using an easy-spin total RNA extraction kit
|
| Label |
biotin
|
| Label protocol |
The sense cDNA was then fragmented and biotin-labeled with TdT (terminal deoxynucleotidyl transferase) using the GeneChip WT Terminal labeling kit
|
| |
|
| Hybridization protocol |
Approximately 5.5 μg of labeled DNA target was hybridized to the Affymetrix GeneChip Array at 45°C for 16hour
|
| Scan protocol |
Hybridized arrays were washed and stained on a GeneChip Fluidics Station 450 and scanned on a GCS3000 Scanner (Affymetrix).
|
| Data processing |
The probe cell intensity data computation and a CEL file generation was performed using Affymetrix® GeneChip Command Console® Software (AGCC).
Affymetrix Power Tools (affymetrix-power-tools.html) with RMA using R (https://www.r-project.org/)
|
| |
|
| Submission date |
Jan 31, 2023 |
| Last update date |
Jan 22, 2024 |
| Contact name |
Jihye Kim |
| E-mail(s) |
[email protected]
|
| Phone |
7209755448
|
| Organization name |
Cleveland Clinic
|
| Street address |
9500 Euclid Ave.
|
| City |
Cleveland |
| State/province |
Ohio |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platform ID |
GPL19251 |
| Series (1) |
| GSE224187 |
Enhancement of the anticancer ability of natural killer cells through allogeneic mitochondrial transfer |
|
