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Sample GSM7099429 Query DataSets for GSM7099429
Status Public on Sep 06, 2023
Title FP67, CD participants, pair sample, Creeping fat
Sample type SRA
 
Source name Mesenteric fat
Organism Homo sapiens
Characteristics tissue: Mesenteric fat
Sex: Male
disease: Crohn's disease
Extracted molecule total RNA
Extraction protocol RNA was extracted using RNeasy Plus Mini Kit (Qiagen, Tokyo, Japan).
The sequencing libraries were prepared with the Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Globin (Illumina, San Diego, CA, United States).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description FP67
Data processing The RNA sequencing reads were trimmed using Cutadapt (v.3.7) in paired-end mode (parameter- Quality : 20 minimum length : 20).
The filtered reads were mapped to the reference genome GRCH38 using STAR software v2.7.9a (parameter- default).
Quantification of the gene expression levels was performed using RNA-SeQC v2.1.2 (parameter- default).
Assembly: GRCH38
Supplementary files format and content: TPM_matrix.txt: Tab-delimited text file includes TPM values for each Sample.
 
Submission date Mar 14, 2023
Last update date Sep 06, 2023
Contact name SoJung Park
E-mail(s) [email protected]
Organization name Ulsan medical university
Street address 88, Olympic-ro 43-gil
City Seoul
State/province Songpa-gu
ZIP/Postal code 05505
Country South Korea
 
Platform ID GPL16791
Series (1)
GSE227376 Transcriptomic analysis reveals the dynamic changes of immune responses during the development of creeping fat in Crohn's disease

Supplementary data files not provided
Processed data are available on Series record
Raw data not provided for this record

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