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Status |
Public on Nov 21, 2011 |
Title |
FOLFOX non-responder training, #1111 |
Sample type |
RNA |
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Source name |
tumor sample of patients with unresectable CRC before mFOLFOX6 therapy
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Organism |
Homo sapiens |
Characteristics |
lesion: Metastasis location: Liver mfolfox6: non-responder gender: M type: training
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Treatment protocol |
Surgical specimens were immediately cut into small pieces after resection, snap frozen in liquid nitrogen, and stored at –80°C
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
Biotin
|
Label protocol |
Experimental procedures for GeneChip(TM) were performed in accordance with the GeneChip Expression Analysis Technical Manual (Affymetrix, Santa Clara, CA), using 3 µg of total RNA.
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Hybridization protocol |
Hybridization was performed according to standard protocols for Affymetrix HG-U133 plus 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
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Data processing |
The data were analyzed with Affymetrix Power Tools (APT version:1.12.0). The apt-probeset-summarize command were used with -a plier-mm-sketch option.
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Submission date |
Apr 19, 2011 |
Last update date |
Nov 21, 2011 |
Contact name |
Shingo Tsuji |
E-mail(s) |
[email protected]
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Organization name |
The University of Tokyo
|
Department |
Research Center for Advanced Science and Technology
|
Lab |
Genome Science div.
|
Street address |
4-6-1 Komaba
|
City |
Meguro-ku |
State/province |
Tokyo |
ZIP/Postal code |
153-8904 |
Country |
Japan |
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|
Platform ID |
GPL570 |
Series (1) |
GSE28702 |
CRC samples for FOLFOX therapy prediction |
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