|
| Status |
Public on Nov 21, 2011 |
| Title |
FOLFOX non-responder training, #1315 |
| Sample type |
RNA |
| |
|
| Source name |
tumor sample of patients with unresectable CRC before mFOLFOX6 therapy
|
| Organism |
Homo sapiens |
| Characteristics |
lesion: Primary
location: Sigmoid
mfolfox6: non-responder
gender: F
type: training
|
| Treatment protocol |
Surgical specimens were immediately cut into small pieces after resection, snap frozen in liquid nitrogen, and stored at –80°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Experimental procedures for GeneChip(TM) were performed in accordance with the GeneChip Expression Analysis Technical Manual (Affymetrix, Santa Clara, CA), using 3 µg of total RNA.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to standard protocols for Affymetrix HG-U133 plus 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
|
| Data processing |
The data were analyzed with Affymetrix Power Tools (APT version:1.12.0). The apt-probeset-summarize command were used with -a plier-mm-sketch option.
|
| |
|
| Submission date |
Apr 19, 2011 |
| Last update date |
Nov 21, 2011 |
| Contact name |
Shingo Tsuji |
| E-mail(s) |
[email protected]
|
| Organization name |
The University of Tokyo
|
| Department |
Research Center for Advanced Science and Technology
|
| Lab |
Genome Science div.
|
| Street address |
4-6-1 Komaba
|
| City |
Meguro-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
153-8904 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28702 |
CRC samples for FOLFOX therapy prediction |
|
