|
| Status |
Public on Oct 01, 2011 |
| Title |
bone marrow cells, 48h stimulated with Flt3-L rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Flt3_L stimulation for 48h
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 8-12 weeks
tissue: bone marrow cells
|
| Treatment protocol |
CsA (Sigma) was added to some cultures at 2 µg/ml for 30 min prior to addition of Flt3-L and was maintained throughout the culture.
|
| Growth protocol |
Bone marrow cells harvested from femurs and tibiae of 8-12 week old C57BL/6 mice were cultured in Iscove’s modified Dulbecco’s medium (containing 10% heat-inactivated FCS (Life Technologies), penicillin 100U/ml, streptomycin 100 ug/ml and Flt3-L ~200 ng/ml (from transfected B16 tumor cells (51)) at 2.5x 106 cells/ml.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
WT Expression Kit (Ambion/Affymetrix) were used. ssDNA was prepared, fragmented and labelled according to the Affymetrix protocol
|
| |
|
| Hybridization protocol |
Fragmented ssDNAs were hybridized to the standard arrays for 17 hours at 45°C; the arrays were then washed and stained using the fluidics station.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000
|
| Data processing |
Microarray data handling was mainly done using Bioconductor 2.6 and an R package AMDA. The Robust Multichip Average (RMA) method was employed to calculate probe set intensity.
|
| |
|
| Submission date |
May 05, 2011 |
| Last update date |
Oct 01, 2011 |
| Contact name |
jinmiao chen |
| Organization name |
Singapore Immunology Network
|
| Street address |
Biopolis
|
| City |
Singapore |
| ZIP/Postal code |
138648 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE29094 |
Calcineurin/NFAT signaling inhibits myeloid hematopoiesis in mice. |
|
