|
| Status |
Public on Jul 12, 2011 |
| Title |
rhoGFP PN4 retinal cells positive FAC sorted biological repliacte 3 |
| Sample type |
RNA |
| |
|
| Source name |
retina of PN4 rhoGFP mice, FAC-sorted
|
| Organism |
Mus musculus |
| Characteristics |
genotype: rhoGFP
cell type: rhoGFP positive retinal cell
genetic background: C57BL/6J-129/Ola
age: postnatal day 4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Qiagen RNeasy Mini Kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to Ambion protocol from 200ng total RNA (MessageAmpTM II-Biotin Enhanced Kit, 2009, Ambion).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were analyzed with GeneSpring GX 11.0.1 software (Agilent) using normalization algorithm RMA.
|
| |
|
| Submission date |
May 16, 2011 |
| Last update date |
Jul 12, 2011 |
| Contact name |
Kai Postel |
| Organization name |
DFG-Center for Regenerative Therapies Dresden
|
| Street address |
Fiedlerstraße 42
|
| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE29318 |
Expression profile of FAC-sorted murine retinal cells |
|
