This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki ([email protected]; 501 Smyth Road) for analysis. Stembase Experiment ID: E113 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=113 SCGP Sample ID: S241 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=113#SAMPLE_202 Short description: 9 days of an 11-point time course study of V6.5 embryonic stem cells and their cognate differentiated embryoid bodies. Time course: 0h, 6h, 12h, 18h, 24h, 36h, 48h, 4 days, 7days, 9 days and 15 days. All samples in triplicate. The cells are differentiating EBs from murine Embryonic Stem Cells, Mouse strain 129 crossed with C67BL/6 isolate V6.5 (P18), obtained from Dr. Josee Coulombe in Dr. M. Rudnicki's lab. Estimated purity: 100% RNA concentration: 2.0 ug/ul Num cells for RNA prep: Unable to break up embryoid bodies to do a cell count. Sample volume: 15 ul
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
