|
| Status |
Public on Dec 31, 2011 |
| Title |
RHT stem at T24, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
rice stem of tillering stage seedlings RHT variety
|
| Organism |
Oryza sativa |
| Characteristics |
tissue: stem
strain: Rathu Heenati
phenotype: resistant variety to brown planthopper
developmental stage: tillering stage
infestation: brown planthopper
time: 24h
|
| Treatment protocol |
The 2nd to 3rd instar nymphs of BPH were transferred to tillering stage seedings (10 BPH nymphs per plant) in a box covered with nylon-mesh.
|
| Growth protocol |
BPH susceptible and resistant rice varieties of TN1（Taichung Native 1） and Rathu Heenati （RHT）were used in this study. The pre-germinated seeds were sown in a plastic tray ( 60 × 45 × 45 cm) and were grown at 28℃/23℃（14h-day and 10h-night cycles）
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (3 ivt express kit manual,Ver4.0, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000: Affymetrix.
|
| Description |
Expression data from rice after brown planthopper attack
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jun 14, 2011 |
| Last update date |
Dec 31, 2011 |
| Contact name |
Yubing Wang |
| E-mail(s) |
[email protected]
|
| Phone |
86-24-54924217
|
| Organization name |
Institute of Plant Physiology and Ecology
|
| Street address |
300 fenglin road
|
| City |
shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE29967 |
Expression data from rice after brown planthopper attack |
|
