|
| Status |
Public on May 24, 2023 |
| Title |
set1Dmst2D_plusTet_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
cultures
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
tissue: cultures
genotype: set1Dmst2D
treatment: +Tet
|
| Treatment protocol |
+Tet cultures were supplemented with tetracycline
|
| Growth protocol |
YEA, grown to log phase at 32C
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Hot phenol extraction followed by DNAse I treatment and Qiagen RNeasy Column Cleanup
RNAseq libraries were prepared by Novogene Corporation Inc.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
KR2015_plusT_1
|
| Data processing |
sequences were trimmed with Trimmomatic, then aligned and quantified by STAR
downstream diff. expression quantification was performed with USEQ DRDS
Assembly: ASM294v2.29
Supplementary files format and content: Excel xlsx files contain raw transcript count matricies
|
| |
|
| Submission date |
May 24, 2023 |
| Last update date |
May 25, 2023 |
| Contact name |
Ajay Larkin |
| E-mail(s) |
[email protected]
|
| Organization name |
Brandeis University
|
| Department |
MCB
|
| Lab |
Ragunathan Lab
|
| Street address |
415 South St
|
| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
02453 |
| Country |
USA |
| |
|
| Platform ID |
GPL28961 |
| Series (1) |
| GSE233333 |
The establishment of HP1-independent heterochromatin reveals an essential role for HP1 proteins in maintaining epigenetic memory. |
|
| Relations |
| BioSample |
SAMN35347407 |
| SRA |
SRX20504727 |
