|
| Status |
Public on Jun 27, 2023 |
| Title |
tcf-repressed sample, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Postmitotic neural progenitors during early differentiation expression Tcf repressor
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: neural tube at the level of the spinal cord
genotype: wild-type
age: 4 days of incubation
|
| Treatment protocol |
Chick embryos were electroporated with column purified plasmid DNA in H2O containing Fast Green (0.5 µg/µl). Briefly, plasmid DNA was injected into the lumen of HH12 (E2) or HH18 (E3) NTs, electrodes were placed on either side of the NT, and electroporation was carried out by applying five 50 ms square pulses using an Intracel Dual Pulse (TSS10) electroporator set at 25 V. Transfected embryos were allowed to develop to the specific stages and then dissected under a fluorescence dissecting microscope.
|
| Growth protocol |
Eggs from White-Leghorn chickens were incubated at 37.8 °C in an atmosphere of 45% humidity and the embryos were staged according to Hamburger and Hamilton (HH: (Hamburger and Hamilton, 1992).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After cell sorting, RNA was extracted by RNeasy Micro Kit (Quiagen 74004) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA were prepared according to WTA2 protocold following the Affymetrix instructions for GeneChip Chicken Genome Array.
|
| |
|
| Hybridization protocol |
Hybridization were performed according to the standard Affymetrix protocol for GeneChip Chicken Genome Array.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 7G (Affymetrix).
|
| Description |
Gene expression data from post-mitotic neural progenitors of chicken embryos expressing a Tcf repressor
|
| Data processing |
The data were analyzed with RMA algorithm using NetAffx Analysis Center.
|
| |
|
| Submission date |
Jun 08, 2023 |
| Last update date |
Jun 27, 2023 |
| Contact name |
Antonio Herrera |
| E-mail(s) |
[email protected]
|
| Organization name |
IBMB-CSIC
|
| Street address |
Baldiri i Reixac
|
| City |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE234518 |
Expression data of Tubb3+ cells from E4 chicken neural tube |
|
