|
| Status |
Public on Jul 11, 2023 |
| Title |
mst2D epe1-deg,+TN 72hrs, rep1 |
| Sample type |
SRA |
| |
|
| Source name |
mst2D epe1-deg 72hr rep1
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
genotype: ade6::ade6+-adh15prom-skp1-OsTIR1-natMX6-adh15prom-skp1-AtTIR1-2NLS nmt81-epe1-3xFLAG-AID mst2D
treatment: +TN
|
| Treatment protocol |
+TN cultures were treated with 15mM thiamine and 500mM 1-napthaleneacetic acid
|
| Growth protocol |
EMMC, grown to log phase at 32C
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Hot phenol extraction followed by DNAse I treatment and Qiagen RNeasy Column Cleanup
RNAseq libraries were prepared by Novogene Corporation Inc.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
sequences were trimmed with Trimmomatic, then aligned and quantified by STAR
downstream diff. expression quantification was performed with USEQ DRDS
Assembly: ASM294v2.29
Supplementary files format and content: Excel xlsx files contain raw transcript count matricies
|
| |
|
| Submission date |
Jun 26, 2023 |
| Last update date |
Jul 11, 2023 |
| Contact name |
Ajay Larkin |
| E-mail(s) |
[email protected]
|
| Organization name |
Brandeis University
|
| Department |
MCB
|
| Lab |
Ragunathan Lab
|
| Street address |
415 South St
|
| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
02453 |
| Country |
USA |
| |
|
| Platform ID |
GPL28961 |
| Series (2) |
| GSE235807 |
Mapping the dynamics of epigenetic adaptation during heterochromatin misregulation [RNA-seq] |
| GSE235808 |
Mapping the dynamics of epigenetic adaptation during heterochromatin misregulation |
|
| Relations |
| BioSample |
SAMN35989938 |
| SRA |
SRX20779378 |
