|
| Status |
Public on Dec 11, 2011 |
| Title |
150c |
| Sample type |
RNA |
| |
|
| Source name |
Nasal lavage cells obtained 7-14 days after acute exacerbation
|
| Organism |
Homo sapiens |
| Characteristics |
phenotype: Asthmatic child with Picornavirus-induced exacerbation
cell type: nasal lavage
|
| Treatment protocol |
Nasal lavage cells were collected in sterile 3 % saline, processed by vigorous pipetting, and stablized in RNALater.
|
| Growth protocol |
Asthmatic children were instructed to visit the study center within 24 h of experiencing symptoms of an exacerbation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from nasal lavage cells employing TRIzol followed by RNeasy.
|
| Label |
biotin
|
| Label protocol |
The samples were labelled according to standardized protocols and reagents from Affymetrix.
|
| |
|
| Hybridization protocol |
The samples were hybridized to HuGene ST1.0 microarrays according to standardized protocols and reagents from Affymetrix.
|
| Scan protocol |
The samples were scanned on a GeneChip® Scanner 3000 7G (Affymetrix).
|
| Description |
Nasal lavage cells obtained 7-14 days post virus-induced exacerbation from asthmatic child 150
|
| Data processing |
The microarray data were preprocessed in Expression Console software (Affymetrix) employing the probe logarithmic intensity error algorithm (parameters - PM-GCBG background subtraction, quantile normalization, iterPLIER summarization).
|
| |
|
| Submission date |
Jun 30, 2011 |
| Last update date |
Dec 11, 2011 |
| Contact name |
Anthony Bosco |
| E-mail(s) |
[email protected]
|
| Organization name |
Telethon Kids Institute
|
| Street address |
100 Roberts Road
|
| City |
Subiaco |
| State/province |
WA |
| ZIP/Postal code |
6008 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE30326 |
Virus-induced inflammatory gene networks underlying asthma exacerbations in vivo |
|
