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Sample GSM76353 Query DataSets for GSM76353
Status Public on Jul 21, 2006
Title DIA-t10; transgenic diaphragm 10 month
Sample type RNA
 
Source name diaphram muscle
Organism Mus musculus
Characteristics Transgenic, snap frozen, diaphragm muscle, 10 month age animal
Extracted molecule total RNA
Extraction protocol Standard Trizol protocol extraction. Total RNA pools from 3-5 mice were processed and hybridized to each microarray.
Label biotin
Label protocol Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Transgenic for alpha tropomyosin slow, snap frozen, diaphragm muscle,10 month age animal
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Sep 29, 2005
Last update date Jul 20, 2006
Contact name Despina Sanoudou
E-mail(s) [email protected]
Phone +30-210-6597453
Fax +30-210-6597545
Organization name Foundation for Biomedical Research, Academy of Athens
Department Molecular Biology
Lab Sanoudou Lab
Street address Soranou Efesiou 4
City Athens
ZIP/Postal code 115 27
Country Greece
 
Platform ID GPL81
Series (1)
GSE3384 Nemaline myopathy mouse model

Data table header descriptions
ID_REF Affymetrix identification codes for each probe set
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
AFFX-MurIL2_at 65.1 A
AFFX-MurIL10_at 224.6 A
AFFX-MurIL4_at 39.2 A
AFFX-MurFAS_at 350.6 A
AFFX-BioB-5_at 1245.3 P
AFFX-BioB-M_at 1632.2 P
AFFX-BioB-3_at 1082.7 P
AFFX-BioC-5_at 5368.7 P
AFFX-BioC-3_at 3420.6 P
AFFX-BioDn-5_at 4890.6 P
AFFX-BioDn-3_at 23636.7 P
AFFX-CreX-5_at 43050.7 P
AFFX-CreX-3_at 71913.1 P
AFFX-BioB-5_st 57.6 A
AFFX-BioB-M_st 49.4 A
AFFX-BioB-3_st 67.5 A
AFFX-BioC-5_st 61.5 A
AFFX-BioC-3_st 34.8 A
AFFX-BioDn-5_st 934 P
AFFX-BioDn-3_st 410.2 A

Total number of rows: 12488

Table truncated, full table size 219 Kbytes.




Supplementary file Size Download File type/resource
GSM76353.CEL.gz 2.9 Mb (ftp)(http) CEL
GSM76353.EXP.gz 504 b (ftp)(http) EXP

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