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Sample GSM76354 Query DataSets for GSM76354
Status Public on Jul 21, 2006
Title DIA-t10-2; transgenic diaphragm 10 month
Sample type RNA
 
Source name diaphram muscle
Organism Mus musculus
Characteristics Transgenic, snap frozen, diaphragm muscle, 10 month age animal
Extracted molecule total RNA
Extraction protocol Standard Trizol protocol extraction. Total RNA pools from 3-5 mice were processed and hybridized to each microarray.
Label biotin
Label protocol Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Transgenic for alpha tropomyosin slow, snap frozen, diaphragm muscle,10 month age animal
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Sep 29, 2005
Last update date Jul 20, 2006
Contact name Despina Sanoudou
E-mail(s) [email protected]
Phone +30-210-6597453
Fax +30-210-6597545
Organization name Foundation for Biomedical Research, Academy of Athens
Department Molecular Biology
Lab Sanoudou Lab
Street address Soranou Efesiou 4
City Athens
ZIP/Postal code 115 27
Country Greece
 
Platform ID GPL81
Series (1)
GSE3384 Nemaline myopathy mouse model

Data table header descriptions
ID_REF Affymetrix identification codes for each probe set
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
AFFX-MurIL2_at 90.9 A
AFFX-MurIL10_at 73.2 A
AFFX-MurIL4_at 20.2 A
AFFX-MurFAS_at 467.8 M
AFFX-BioB-5_at 4424.8 P
AFFX-BioB-M_at 10479.8 P
AFFX-BioB-3_at 4914.1 P
AFFX-BioC-5_at 17456.1 P
AFFX-BioC-3_at 12761 P
AFFX-BioDn-5_at 16254.2 P
AFFX-BioDn-3_at 60491.5 P
AFFX-CreX-5_at 152548.8 P
AFFX-CreX-3_at 195991.3 P
AFFX-BioB-5_st 582.1 A
AFFX-BioB-M_st 247.7 A
AFFX-BioB-3_st 388.6 A
AFFX-BioC-5_st 55.2 A
AFFX-BioC-3_st 124 A
AFFX-BioDn-5_st 691.6 A
AFFX-BioDn-3_st 885.6 P

Total number of rows: 12488

Table truncated, full table size 219 Kbytes.




Supplementary file Size Download File type/resource
GSM76354.CEL.gz 2.7 Mb (ftp)(http) CEL
GSM76354.EXP.gz 266 b (ftp)(http) EXP

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