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Sample GSM76366 Query DataSets for GSM76366
Status Public on Jul 21, 2006
Title GAS-t7-2; transgenic gastrocnemus 7 month
Sample type RNA
 
Source name gastrocnemus muscle
Organism Mus musculus
Characteristics Transgenic, snap frozen, gastrocnemus muscle, 7month age animal
Extracted molecule total RNA
Extraction protocol Standard Trizol protocol extraction. Total RNA pools from 3-5 mice were processed and hybridized to each microarray.
Label biotin
Label protocol Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Transgenic for alpha tropomyosin slow, snap frozen, gastrocnemus muscle, 7month age animal
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Sep 29, 2005
Last update date Jul 20, 2006
Contact name Despina Sanoudou
E-mail(s) [email protected]
Phone +30-210-6597453
Fax +30-210-6597545
Organization name Foundation for Biomedical Research, Academy of Athens
Department Molecular Biology
Lab Sanoudou Lab
Street address Soranou Efesiou 4
City Athens
ZIP/Postal code 115 27
Country Greece
 
Platform ID GPL81
Series (1)
GSE3384 Nemaline myopathy mouse model

Data table header descriptions
ID_REF Affymetrix identification codes for each probe set
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
AFFX-MurIL2_at 57.9 A
AFFX-MurIL10_at 74.3 A
AFFX-MurIL4_at 42.3 A
AFFX-MurFAS_at 50.8 A
AFFX-BioB-5_at 1048.8 P
AFFX-BioB-M_at 1377.6 P
AFFX-BioB-3_at 715.6 P
AFFX-BioC-5_at 4243.2 P
AFFX-BioC-3_at 2754.8 P
AFFX-BioDn-5_at 3091.7 P
AFFX-BioDn-3_at 20411.1 P
AFFX-CreX-5_at 34763 P
AFFX-CreX-3_at 63105.5 P
AFFX-BioB-5_st 284.1 A
AFFX-BioB-M_st 101.7 A
AFFX-BioB-3_st 119.8 A
AFFX-BioC-5_st 63 A
AFFX-BioC-3_st 75.2 A
AFFX-BioDn-5_st 1058.4 A
AFFX-BioDn-3_st 629.4 A

Total number of rows: 12488

Table truncated, full table size 219 Kbytes.




Supplementary file Size Download File type/resource
GSM76366.CEL.gz 2.9 Mb (ftp)(http) CEL
GSM76366.EXP.gz 266 b (ftp)(http) EXP

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