|
| Status |
Public on Jul 26, 2012 |
| Title |
1016-00013_LS |
| Sample type |
RNA |
| |
|
| Source name |
skin biopsy, LS
|
| Organism |
Homo sapiens |
| Characteristics |
subject: 1016-00013
disease state: moderate-to-severe psoriasis
tissue: skin
biopsy type: psoriasis lesion (LS)
|
| Treatment protocol |
For each patient, one 4-mm skin punch biopsy was collected from a lesion located on the trunk or extremities, with approximately 0.5 mm or more of induration and approximately 5 mm from the edge of the plaque, and another from a macroscopically normal non-lesion on a similar body area to the biopsy plaque (i.e. arm, arm or leg, leg). Biopsies were kept frozen at -80oC until RNA preparation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Qiagen's RNeasy mini kit according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 11 microg of cRNA were hybridized for 16 hr at 45oC on a GeneChip HG U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
P126504_LJ_R43975_81
|
| Data processing |
The data was normalized by GCRMA.
|
| |
|
| Submission date |
Jul 27, 2011 |
| Last update date |
Jul 26, 2012 |
| Contact name |
K Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Janssen R&D
|
| Street address |
1400 McKean Road
|
| City |
Spring House |
| State/province |
PA |
| ZIP/Postal code |
19477 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE30999 |
Expression data from skin biopsy samples from patients with moderate-to-severe psoriasis |
|
