|
| Status |
Public on Sep 25, 2023 |
| Title |
AdipoKO_BAT-2 251486841534_1_4 |
| Sample type |
RNA |
| |
|
| Source name |
Brown Adipose Tissue
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Adipose tissue
Sex: Male
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the Qiagen RNeasy Kit for total RNA isolation
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
AdipoKO_BAT-2
Gene expression from brown adipose tissue GR knockout mice
|
| Data processing |
The Agilent Feature Extraction Software performed error modeling, adjusting for additive and multiplicative noise
|
| |
|
| Submission date |
Aug 24, 2023 |
| Last update date |
Sep 25, 2023 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (2) |
| GSE241651 |
Glucocorticoid receptor signaling in adipose tissue I |
| GSE241653 |
Glucocorticoid receptor signaling in adipose tissue |
|
