|
| Status |
Public on Sep 06, 2011 |
| Title |
DB94 |
| Sample type |
RNA |
| |
|
| Source name |
Lung adenocarcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
egfr status: Wild-Type
gender: Female
Sex: F
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single isolates of tumor samples were homogenized in the presence of Trizol reagent and total cellular RNA was purified according to manufacturer’s procedures. RNA samples were further purified using RNeasy spin columns
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to standard Affymetrix protocol from 5 microgram total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
|
| Data processing |
Quantile normalization and RMA were done with RMAExpress (http://rmaexpress.bmbolstad.com/)
|
| |
|
| Submission date |
Aug 19, 2011 |
| Last update date |
Sep 06, 2011 |
| Contact name |
Luc Girard |
| E-mail(s) |
[email protected]
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Hamon Center for Therapeutic Oncology Research
|
| Lab |
NB8.114A
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-8593 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE31546 |
UMCCC Primary Lung Cancer Specimens |
| GSE31908 |
Primary Lung Cancer Specimens |
|
