|
| Status |
Public on Sep 06, 2011 |
| Title |
27NL(B) |
| Sample type |
RNA |
| |
|
| Source name |
Normal lung
|
| Organism |
Homo sapiens |
| Characteristics |
egfr status: Wild-type
age: 74
race: WNH
Sex: M
smoking: Former
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to standard Affymetrix protocol from 5 microgram total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
|
| Data processing |
Quantile normalization and RMA were done with RMAExpress (http://rmaexpress.bmbolstad.com/)
|
| |
|
| Submission date |
Aug 19, 2011 |
| Last update date |
Sep 06, 2011 |
| Contact name |
Luc Girard |
| E-mail(s) |
[email protected]
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Hamon Center for Therapeutic Oncology Research
|
| Lab |
NB8.114A
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-8593 |
| Country |
USA |
| |
|
| Platform ID |
GPL97 |
| Series (2) |
| GSE31548 |
MSKCC-B Primary Lung Cancer Specimens |
| GSE31908 |
Primary Lung Cancer Specimens |
|
