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Status
Public on Mar 29, 2006
Title
Expression data of rectal cancer T54
Sample type
RNA
Source name
Patient ID T54
Organism
Homo sapiens Characteristics
Tissue: rectal cancer
Extracted molecule
total RNA
Extraction protocol
Surgically isolated human colorectal cancer tissues were immediately frozen in liquid nitrogen. Tissues were lysed and total RNA was extracted using the Sepasol-RNA I (WAKO, Osaka, Japan) according to the manufacturer's instructions. Total RNA was then purified using an RNeasy column (Qiagen, Austin, TX).
Label
biotin
Label protocol
Approximately 5 ug of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
training-responder
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Oct 21, 2005
Last update date
Mar 16, 2009
Contact name
Toshiaki Watanabe
Phone
81-3-5800-8653
Organization name
University of Tokyo
Department
Department of Surgical Oncology
Street address
7-3-1, Hongo
City
Bunkyo-ku
State/province
Tokyo
ZIP/Postal code
113-8655
Country
Japan
Platform ID
GPL8300 Series (1)
GSE3493 Rectal Cancer Cells and Radiation-sensitivity
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
Data table ID_REF VALUE ABS_CALL 100_g_at
123.1
A
1000_at
1590.2
P
1001_at
296.4
A
1002_f_at
90
A
1003_s_at
377.7
A
1004_at
390.8
A
1005_at
312.4
A
1006_at
52.1
A
1007_s_at
1706.9
P
1008_f_at
7105.8
P
1009_at
1537.2
P
101_at
84.8
A
1010_at
72.4
A
1011_s_at
363.8
P
1012_at
28
A
1013_at
149.1
A
1014_at
717.1
A
1015_s_at
118.7
A
1016_s_at
49
A
1017_at
79.5
A
12625 206 Kbytes .Supplementary data files not provided
