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Status
Public on Mar 29, 2006
Title
Expression data of rectal cancer T43
Sample type
RNA
Source name
Patient ID T43
Organism
Homo sapiens
Characteristics
Tissue: rectal cancer
Extracted molecule
total RNA
Extraction protocol
Surgically isolated human colorectal cancer tissues were immediately frozen in liquid nitrogen. Tissues were lysed and total RNA was extracted using the Sepasol-RNA I (WAKO, Osaka, Japan) according to the manufacturer's instructions. Total RNA was then purified using an RNeasy column (Qiagen, Austin, TX).
Label
biotin
Label protocol
Approximately 5 ug of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
training-non-responder
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Oct 21, 2005
Last update date
Mar 16, 2009
Contact name
Toshiaki Watanabe
Phone
81-3-5800-8653
Organization name
University of Tokyo
Department
Department of Surgical Oncology
Street address
7-3-1, Hongo
City
Bunkyo-ku
State/province
Tokyo
ZIP/Postal code
113-8655
Country
Japan
Platform ID
GPL8300
Series (1)
GSE3493
Rectal Cancer Cells and Radiation-sensitivity
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
Data table
ID_REF
VALUE
ABS_CALL
100_g_at
472.3
A
1000_at
1258.6
P
1001_at
129.2
A
1002_f_at
40.7
A
1003_s_at
270.9
A
1004_at
412.5
A
1005_at
1368.8
P
1006_at
16.8
A
1007_s_at
2032.1
P
1008_f_at
6927.2
P
1009_at
3310.5
P
101_at
44.2
A
1010_at
57.9
A
1011_s_at
715
P
1012_at
12.4
A
1013_at
44
A
1014_at
464.9
A
1015_s_at
56.7
A
1016_s_at
75.4
A
1017_at
58.9
A
Total number of rows: 12625 Table truncated, full table size 205 Kbytes .Supplementary data files not provided