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Status
Public on Mar 29, 2006
Title
Expression data of rectal cancer T46
Sample type
RNA
Source name
Patient ID T46
Organism
Homo sapiens
Characteristics
Tissue: rectal cancer
Extracted molecule
total RNA
Extraction protocol
Surgically isolated human colorectal cancer tissues were immediately frozen in liquid nitrogen. Tissues were lysed and total RNA was extracted using the Sepasol-RNA I (WAKO, Osaka, Japan) according to the manufacturer's instructions. Total RNA was then purified using an RNeasy column (Qiagen, Austin, TX).
Label
biotin
Label protocol
Approximately 5 ug of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
training-non-responder
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Oct 21, 2005
Last update date
Mar 16, 2009
Contact name
Toshiaki Watanabe
Phone
81-3-5800-8653
Organization name
University of Tokyo
Department
Department of Surgical Oncology
Street address
7-3-1, Hongo
City
Bunkyo-ku
State/province
Tokyo
ZIP/Postal code
113-8655
Country
Japan
Platform ID
GPL8300
Series (1)
GSE3493
Rectal Cancer Cells and Radiation-sensitivity
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
Data table
ID_REF
VALUE
ABS_CALL
100_g_at
272.8
A
1000_at
903.6
P
1001_at
67.5
A
1002_f_at
45.4
A
1003_s_at
62.2
A
1004_at
134.8
A
1005_at
262.6
A
1006_at
4.6
A
1007_s_at
1705.6
P
1008_f_at
5180.3
P
1009_at
4426.7
P
101_at
113.4
A
1010_at
20.2
A
1011_s_at
512.6
P
1012_at
16.4
A
1013_at
57.1
P
1014_at
497.4
A
1015_s_at
72.2
A
1016_s_at
40.1
A
1017_at
104.9
P
Total number of rows: 12625 Table truncated, full table size 205 Kbytes .Supplementary data files not provided