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Status
Public on Mar 29, 2006
Title
Expression data of rectal cancer T70
Sample type
RNA
Source name
Patient ID T70
Organism
Homo sapiens Characteristics
Tissue: rectal cancer
Extracted molecule
total RNA
Extraction protocol
Surgically isolated human colorectal cancer tissues were immediately frozen in liquid nitrogen. Tissues were lysed and total RNA was extracted using the Sepasol-RNA I (WAKO, Osaka, Japan) according to the manufacturer's instructions. Total RNA was then purified using an RNeasy column (Qiagen, Austin, TX).
Label
biotin
Label protocol
Approximately 5 ug of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
training-non-responder
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Oct 21, 2005
Last update date
Mar 16, 2009
Contact name
Toshiaki Watanabe
Phone
81-3-5800-8653
Organization name
University of Tokyo
Department
Department of Surgical Oncology
Street address
7-3-1, Hongo
City
Bunkyo-ku
State/province
Tokyo
ZIP/Postal code
113-8655
Country
Japan
Platform ID
GPL8300 Series (1)
GSE3493 Rectal Cancer Cells and Radiation-sensitivity
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
Data table ID_REF VALUE ABS_CALL 100_g_at
405.7
A
1000_at
1806.6
P
1001_at
197.3
A
1002_f_at
80.6
A
1003_s_at
377.7
A
1004_at
255.2
A
1005_at
318
A
1006_at
53.7
A
1007_s_at
1408
P
1008_f_at
6817
P
1009_at
1784.4
P
101_at
53.5
A
1010_at
39.5
A
1011_s_at
426.7
P
1012_at
15.2
A
1013_at
93.7
A
1014_at
555.3
A
1015_s_at
145.9
A
1016_s_at
67.2
A
1017_at
76.6
A
12625 205 Kbytes .Supplementary data files not provided
