|
| Status |
Public on Feb 16, 2012 |
| Title |
WholeBody_for_lens_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Whole embryo
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Whole embryo
Stage: E10.5-12.5
|
| Treatment protocol |
Wild type lens or whole embryonic tissue dissected in 1XPBS from mouse ICR strain, treated with RNAlater (Qiagen) and stored at -80C until RNA isolation
|
| Growth protocol |
Timed embryos, plug day recorded as E0.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy Mini Kit Extraction Protocol
|
| Label |
biotin
|
| Label protocol |
cRNA biotin labelled via in vitro transcription
|
| |
|
| Hybridization protocol |
16 hr incubation according to Affymetrix protocol
|
| Scan protocol |
Double amplified with phycoerythin-tagged Streptavidin, Excitation 488 nm, emission 570 nm
|
| Description |
embryonic tissue
|
| Data processing |
Raw data are preprocessed using the robust multiarray average (RMA) rma function in the bioconductor package affy version 0.7
|
| |
|
| Submission date |
Sep 23, 2011 |
| Last update date |
Feb 16, 2012 |
| Contact name |
Joshua WK Ho |
| E-mail(s) |
[email protected]
|
| Organization name |
Brigham and Women's Hospital
|
| Department |
Department of Medicine
|
| Lab |
Division of Genetics
|
| Street address |
41 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE32334 |
Developmental timecourse of mouse ocular lens and whole embryo control |
|
