|
| Status |
Public on Oct 07, 2011 |
| Title |
ERa EtOH |
| Sample type |
SRA |
| |
|
| Source name |
H3396 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H3396 cells
treatment: ethanol
comment: Santa Cruz: ERa (HC20)
chip antibody: ERa
chip antibody manufacturer: Santa Cruz
chip antibody catalog #: HC20
|
| Treatment protocol |
Before 1h of estrogen or ethanol treatment, H3396 cells were cultured in charcol stripped medium for 72h
|
| Growth protocol |
H3396 cells were maintained in RPMI 1640 supplemented with 10 mM HEPES, 10% fetal bovine serum (FBS), and antibiotics.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIP assays were performed according to standard protocols
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
Chromatin IP against Estrogen receptor alpha
|
| Data processing |
Alignment: Sequence reads were obtained and mapped to the human (March, 2006) genomes using the Illumina Genome Analyzer Pipeline. All reads mapping with two or fewer mismatches were retained.
|
| |
|
| Submission date |
Sep 23, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Marco Antonio Mendoza-Parra |
| E-mail(s) |
[email protected]
|
| Organization name |
IGBMC
|
| Street address |
1, rue Laurent Fries
|
| City |
Illkirch; Strasbourg |
| ZIP/Postal code |
67400 |
| Country |
France |
| |
|
| Platform ID |
GPL9052 |
| Series (2) |
| GSE32349 |
Methylation specifies distinct estrogen-induced binding site repertoires of CBP to chromatin (ChIP-Seq) |
| GSE32350 |
Methylation specifies distinct estrogen-induced binding site repertoires of CBP to chromatin |
|
| Relations |
| SRA |
SRX100266 |
| BioSample |
SAMN00738208 |
| Named Annotation |
GSM801057_ERa_EtOH_aligned_hg18.bed.gz |
