|
Status |
Public on Apr 09, 2024 |
Title |
RNAseq_keratinocytes_female_Sepi_rep5 |
Sample type |
SRA |
|
|
Source name |
skin
|
Organism |
Mus musculus |
Characteristics |
tissue: skin cell type: keratinocytes genotype: WT female treatment: S. epidermidis
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with the Qiagen microRNA kit following manufactuer's instruction Sequencing library was prepared using the Ovation SoLo RNA-Seq library preparataion kit (Tecan) following manufacture’s instructions.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
RNAseq_DEG_female_Ctrl_vs_Sepi.txt
|
Data processing |
Sequencing reads were mapped to the C57BL/6 mouse genome (GRCm38: mm10) and differential gene expression was calculated by DESeq2 Assembly: mm10 Supplementary files format and content: txt
|
|
|
Submission date |
Jan 15, 2024 |
Last update date |
Apr 09, 2024 |
Contact name |
Verena Link |
E-mail(s) |
[email protected]
|
Organization name |
NIH
|
Department |
NIAID
|
Street address |
4 Memorial Drive
|
City |
Bethesda |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL19057 |
Series (2) |
GSE253293 |
Sexual dimorphism in skin immunity is mediated by androgen-ILC2-dendritic cell axis [Bulk RNA-seq] |
GSE253295 |
Sexual dimorphism in skin immunity is mediated by androgen-ILC2-dendritic cell axis |
|
Relations |
BioSample |
SAMN39447778 |
SRA |
SRX23223788 |