|
| Status |
Public on Apr 09, 2024 |
| Title |
RNAseq_keratinocytes_male_Sepi_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
skin
|
| Organism |
Mus musculus |
| Characteristics |
tissue: skin
cell type: keratinocytes
genotype: WT male
treatment: S. epidermidis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the Qiagen microRNA kit following manufactuer's instruction
Sequencing library was prepared using the Ovation SoLo RNA-Seq library preparataion kit (Tecan) following manufacture’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
RNAseq_DEG_male_Ctrl_vs_Sepi.txt
|
| Data processing |
Sequencing reads were mapped to the C57BL/6 mouse genome (GRCm38: mm10) and differential gene expression was calculated by DESeq2
Assembly: mm10
Supplementary files format and content: txt
|
| |
|
| Submission date |
Jan 15, 2024 |
| Last update date |
Apr 09, 2024 |
| Contact name |
Verena Link |
| E-mail(s) |
[email protected]
|
| Organization name |
NIH
|
| Department |
NIAID
|
| Street address |
4 Memorial Drive
|
| City |
Bethesda |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE253293 |
Sexual dimorphism in skin immunity is mediated by androgen-ILC2-dendritic cell axis [Bulk RNA-seq] |
| GSE253295 |
Sexual dimorphism in skin immunity is mediated by androgen-ILC2-dendritic cell axis |
|
| Relations |
| BioSample |
SAMN39447773 |
| SRA |
SRX23223793 |
