|
| Status |
Public on Nov 01, 2011 |
| Title |
Drosophila_DL1 cells_control RNAi 4 |
| Sample type |
RNA |
| |
|
| Source name |
DL1 cells control RNAi
|
| Organism |
Drosophila melanogaster |
| Characteristics |
days after dsrna bathing: 5d
RNAi: renilla dsRNA
|
| Treatment protocol |
The cells were bathed in dsRNA to renilla or nbr, and grown at 25˚C for five days.
|
| Growth protocol |
DL1 cells were grown at 25˚C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified using TRIzol reagent (Invitrogen)
cDNA was amplifed using Ovation Pico WTA system (NuGEN)
|
| Label |
biotin
|
| Label protocol |
Encore™ Biotin Module (NuGEN)
|
| |
|
| Hybridization protocol |
Encore™ Biotin Module (NuGEN)
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
3766_24271_Renilla4 Drosophila_2_(Drosophila_2)
|
| Data processing |
Normalization of probe signal intensity levels across arrays was performed using the GCRMA algorithm implemented in Partek Genomic suite 6.2 beta.
|
| |
|
| Submission date |
Oct 03, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Bonini Nancy |
| Organization name |
University of Pennsylvania
|
| Street address |
415 S University Ave
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE32564 |
Genes affected upon dsRNA knockdown treatment for nbr/CG9247 in Drosophila DL1 cells and small RNA profiling in Drosophila wild-type and nbr[f02257] mutants |
| GSE32683 |
Genes affected upon dsRNA knockdown treatment for nbr/CG9247 in Drosophila DL1 cells |
|
| Relations |
| Reanalyzed by |
GSE119084 |
