|
| Status |
Public on Apr 03, 2024 |
| Title |
LIM1215 cell line, Reverse (Withdrawal treatment), Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Lim1215_W20
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LIM1215
cell type: Human colorectal cancer cell
treatment: Reverse (Withdrawal treatment)
|
| Treatment protocol |
Parental human CRC cell lines cultured from single clone were seeded overnight in 10 cm dish at 1000000 cells per dish. The following day, 1000000 NK-CD16 cells with 1 μg/ml cetuximab (anti-EGFR) were added for ADCC challenge.
|
| Growth protocol |
LIM1215 cells were cultured in RPMI 1640 medium with L-glutamine, supplemented with 10% FBS, 1 μg/ml hydrocortisone, 1 μg/ml human insulin, 100 U/mL penicillin-streptomycin. SW48 cells were cultured in complete medium: RPMI 1640 medium with L-glutamine, supplemented with 10% FBS and 100U/mL penicillin-streptomycin. Caco-2 cells were cultured with Eagle's MEM, supplemented with 20% FBS and 100 U/mL penicillin-streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was then isolated from cell pellets using RNeasy Plus Mini Kit (QIAGEN) following the instructions.
|
| Label |
biotin
|
| Label protocol |
Briefly, the GeneChip® WT PLUS Reagent Kit (Affymetrix) was used to generate sense-strand cDNA from total RNA (250 ng). Then the cDNA was fragmented and labeled with the kit and was used to prepare a hybridization cocktail with the GeneTitan Hybridization Wash and Stain Kit for WT Arrays (Thermo Fisher).
|
| |
|
| Hybridization protocol |
Processed cDNA was then hybridized to the Human HT Clariom S 24-peg plate arrays, washed, stained.
|
| Scan protocol |
Scanned by the Affymetrix GeneTitan MC Instrument.
GeneChip Command Console Software (AGCC) was used for GeneTitan Instrument control.
|
| Description |
Lim1215, Reverse (Withdrawal treatment) cells, replicate 2
|
| Data processing |
GeneChip Command Console Software (AGCC) was used for GeneTitan Instrument control.
|
| |
|
| Submission date |
Feb 13, 2024 |
| Last update date |
Apr 03, 2024 |
| Contact name |
Yanguang Cao |
| E-mail(s) |
[email protected]
|
| Organization name |
UNC Chapel Hill
|
| Street address |
301 Pharmacy Ln
|
| City |
Chapel Hill |
| State/province |
North Carolina |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL24324 |
| Series (1) |
| GSE255674 |
Gene expression profiling of human colorectal cancer cell lines [Affymetrix] |
|
