|
| Status |
Public on Sep 03, 2019 |
| Title |
Il1a-/- DCa1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse lung mononuclear cells
|
| Organism |
Mus musculus |
| Characteristics |
genotype: wild-type
tissue: CDC11c+ lung mononuclear cells
genetic background: C57BL/6
|
| Biomaterial provider |
The University of Washington (Seattle, WA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from myeloid dendritic cells with TriZol reagent (Invitrogen) according to a standard protocol. RNA integrity of individual samples was confirmed with an Agilent 2100 Bioanalyzer. CD11c+ cells were purified to 97% purify from lung mononuclear cells by two consecutive rounds of magnetic cell separation using CD11c-antibody-labled magnetic beads and LS columns for positive cell selection as per manufacturer’s protocol (Miltenyi Biotech). The purity of CD11c+ cells was verified by flow cytometry prior to pooling CD11c+ cells from three mice for total RNA isolation. Two pools of CD11c+ cells from three mice each were prepared for WT and Il1a-/- experimental groups.
|
| Label |
biotin
|
| Label protocol |
For microarray hybridization, RNA was processed for hybridization to Affymetrix Mouse Gene 1.0 ST Array through a core facility in the Functional Genomics Laboratory in the Center for Ecogenetics and Environmental Health at the University of Washington according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
The standard hybridization protocol was used as recommended by Affymterix.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 through the University of Washington Functional Genomics Laboratory in the Center for Ecogenetics and Environmental Health (http://depts.washington.edu/ceeh/ServiceCores/FC1/FC1.html).
|
| Description |
myeloid dendritic cells
|
| Data processing |
Bioconductor software, RMA normalization log2
|
| |
|
| Submission date |
Oct 04, 2011 |
| Last update date |
Sep 03, 2019 |
| Contact name |
James William MacDonald |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Washington
|
| Department |
Environmental and Occupational Health Sciences
|
| Street address |
4225 Roosevelt Way NE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105-6099 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE32619 |
Host resistance to pulmonary infection with Mycobacterium tuberculosis is mediated by IL-1alpha. |
|
