|
| Status |
Public on Mar 07, 2013 |
| Title |
Control-3 |
| Sample type |
RNA |
| |
|
| Source name |
MIN6 cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell line: MIN6
sample type: sorted cell subpopulations
transfection: negative control siRNA
|
| Treatment protocol |
MIN6 cells were transfected with negative control siRNA (control) or Hlxb9 siRNA (HB9) using the nucleofection method with Solution -T (Amaxa/Lonza), and RNAiMax (Invitrogen). Total RNA was isolated 72 hours post-transfection.
|
| Growth protocol |
MIN6 cells were cultured in DMEM low glucose medium supplemented with 15% FBS and, antibiotic and antimycotic, at 37˚C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNAeasy kit according to the manufacturer's instructions (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
gene expression upon knockdown with negative control siRNA
|
| Data processing |
RMA by software Partek
|
| |
|
| Submission date |
Oct 05, 2011 |
| Last update date |
Mar 07, 2013 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
[email protected]
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE32653 |
Hlxb9 regulated genes in pancreatic beta cells |
|
