|
| Status |
Public on Mar 02, 2024 |
| Title |
Prototype AMI (1) |
| Sample type |
protein |
| |
|
| Source name |
Serum/Plasma
|
| Organism |
Homo sapiens |
| Characteristics |
condition: Prototype Autoimmune Disease Sample
tissue: Serum/Plasma
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
protein |
| Extraction protocol |
Blood was collected from SLE patients and healthy controls and then processed into serum or plasma which was then screened by array.
|
| Label |
R-PE conjugated goat anti-human IgG secondary antibody
|
| Label protocol |
Secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from samples.
|
| |
|
| Hybridization protocol |
A fluorescent secondary antibody was added to a PBS solution containing conjugated beads bound to antibodies.
|
| Scan protocol |
A Luminex FlexMAP 3D instrument was used to quantify autoantibody levels in each sample by measuring fluorescence (in MFI) from each barcoded bead.
|
| Description |
Antibodies in samples bind to barcoded beads conjugated to antigens. Antibody reactivity was quantified by the fluorescence measured as MFI from the secondary antibody.
|
| Data processing |
Barebeed MFI values were subtracted from the MFI values for all analytes in each sample and replicate MFI values were averaged.
ACA Array version 2 Platform Raw MFI_02272024.xlsx: raw data
matrix_MFI.txt: Numerical values represent median fluorescent intensity (MFI).
|
| |
|
| Submission date |
Feb 28, 2024 |
| Last update date |
Mar 02, 2024 |
| Contact name |
Allan Feng |
| E-mail(s) |
[email protected]
|
| Phone |
2055678956
|
| Organization name |
Stanford School of Medicine
|
| Department |
Imm/Rheum
|
| Lab |
Utz
|
| Street address |
269 Campus Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL34255 |
| Series (1) |
| GSE260475 |
Impaired innate and adaptive immune responses to BNT162b2 SARSCoV-2 vaccination in systemic lupus erythematosus [ACA] |
|
