|
| Status |
Public on Sep 06, 2024 |
| Title |
Restricted cow 0078 |
| Sample type |
RNA |
| |
|
| Source name |
Liver biopsy
|
| Organism |
Bos taurus |
| Characteristics |
physiology: early lactation stage, multiparous.
breed: Holstein cow
diet: Nutrient restricted diet
|
| Treatment protocol |
At 24 ± 3 d in milk, multiparous cows were either allowed to continue ad libitum intake of a lactation diet (CON, n = 6), or the ration was diluted with barley straw (48% DM) for 4 d (RES, n = 6). On d 3, one healthy rear mammary quarter was infused with 50 µg of LPS. Liver biopsies were performed 24 h after LPS challenge
|
| Growth protocol |
Twelve cows were studied at earlylactation stage,
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared through homogenization of approximately 50 mg of liver tissue according to manufacture protocol of mirVana miRNA extraction kit (Invitrogen Life Technologies, Cergy Pontoise, France)
|
| Label |
CY3
|
| Label protocol |
100 ng of total RNA were amplified and fluorescently labelled to produce Cy3 cRNA using the Low Input Quick Amp Labeling one colors Kit (Agilent, USA) in the presence of spike-in one color control.
|
| |
|
| Hybridization protocol |
After purification (Qiagen RNeasy Mini Kit), 1,65 µg of Cy3 labeled cRNA were hybridized onto G2519F-023647 Bovine (V2) Gene Expression Microarray, 4x44K (Agilent, USA) with hybridization buffer (Agilent Gene Expression Hybridization Kit) according to the manufacturer’s instructions. After 17h of hybridization, slides were washed with the Gene Expression Wash Pack.
|
| Scan protocol |
Microarrays were scanned with Agilent G2505 scanner.
|
| Description |
Holstein early lactation restricted cow
|
| Data processing |
Data were extracted with Feature Extraction 11,0 software (Agilent, USA) (no background subtraction).
|
| |
|
| Submission date |
Mar 13, 2024 |
| Last update date |
Sep 06, 2024 |
| Contact name |
Christine LEROUX |
| E-mail(s) |
[email protected]
|
| Organization name |
INRAE
|
| Lab |
Christine LEROUX
|
| Street address |
Route de Theix
|
| City |
St Genès-Champanelle |
| ZIP/Postal code |
63122 |
| Country |
France |
| |
|
| Platform ID |
GPL11648 |
| Series (1) |
| GSE261541 |
Liver transcriptome modifications by nutrient restriction in early lactation Holstein cows challenged with intramammary lipopolysaccharide |
|
