|
| Status |
Public on May 20, 2012 |
| Title |
Ex 3 CoVa 120 |
| Sample type |
RNA |
| |
|
| Source name |
COVA transfected, 120 hour timepoint
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: S2
|
| Treatment protocol |
Calcium phosphate transfection for siRNA specific for either GFP or COVA
|
| Growth protocol |
S2 cells cultrued in Schneider's media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction through Trizol followed by Rneasy Cleanup
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr on GeneChip Drosophila Genome 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
Standard Affymetrix scanning
|
| Description |
Replicate 3, COVA transfected, 120 hour timepoint
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. MAS 5.0 output was then entered into dCHIP where the arrays were normalized to the median intensity array.
|
| |
|
| Submission date |
Oct 12, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
William Alexander Freije |
| E-mail(s) |
[email protected]
|
| Phone |
310-924-4194
|
| Organization name |
UCLA
|
| Department |
Obstetrics and Gynecology
|
| Lab |
Freije Laboratory
|
| Street address |
650 Charles Young Drive, South CHS B8-127
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE32912 |
Expression profiling of attenuated mitochondrial function identifies retrograde signals in Drosophila |
|
| Relations |
| Reanalyzed by |
GSE119084 |
