|
| Status |
Public on Jul 11, 2024 |
| Title |
nrde-3 PDPhe replicate 4 |
| Sample type |
SRA |
| |
|
| Source name |
Neurons
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
tissue: Neurons
cell type: ADL neurons
genotype: nrde-3(gg66)
treatment: PDPhe
|
| Growth protocol |
C. elegans ADL neurons from control one-day old adults and postdauer (pheromone) one-day old adults.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction of FACS events was done using NEB Monarch Total RNA Miniprep (New England Biolabs [NEB] T2010).
RNA-Seq libraries were prepared using NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB E7490S) and NEBNext® Ultra™ II RNA Library Prep with Sample Purification Beads (NEB E7775S) following the recommendations of the manufacturer. Indexing of libraries was done using NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB E7335S) and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2) (NEB E7500S) according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
SH321PDPhe_4_CCGTCC
|
| Data processing |
Data analysis was performed on the CLC Genomics Workbench (Qiagen) with differential expression assessed using EdgeR.
Assembly: WS235
Supplementary files format and content: tab-delimited text files contain raw counts and RPKM for each sample
|
| |
|
| Submission date |
May 31, 2024 |
| Last update date |
Jul 11, 2024 |
| Contact name |
Maria C. Ow |
| E-mail(s) |
[email protected]
|
| Organization name |
Syracuse University
|
| Street address |
107 College Dr, LSC117
|
| City |
Syracuse |
| State/province |
NY |
| ZIP/Postal code |
13224 |
| Country |
USA |
| |
|
| Platform ID |
GPL19757 |
| Series (1) |
| GSE268801 |
RNAi-dependent expression of sperm genes in ADL chemosensory neurons is required for olfactory responses in Caenorhabditis elegans |
|
| Relations |
| BioSample |
SAMN41624486 |
| SRA |
SRX24768331 |
