|
| Status |
Public on Apr 19, 2012 |
| Title |
Braf-cko, biological replicate 3, technical replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampus, conditional knockout
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed background (93.7% C57BL/6J, 6.2% FVB, 0.1% 129/Sv)
gender: male
age: 4 months
tissue: hippocampus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Animals were killed with CO2, the complete hippocampal tissue was prepared, and total RNA was extracted with the Trizol protocol. The integrity and quality of the RNA samples were analyzed with an RNA electrophoresis chip (RNA 6000 Nano Kit, Agilent, Boeblingen, Germany).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit (Ambion, Austin, TX, USA) according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The raw data were processed in R using beadarray and vsn for normalization
|
| |
|
| Submission date |
Nov 10, 2011 |
| Last update date |
Apr 19, 2012 |
| Contact name |
Benedikt Wefers |
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Developmental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg/Munich |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE33618 |
MAPK Signaling Determines Anxiety in the Juvenile Mouse Brain but Depression-like Behavior in Adults |
|
