|
| Status |
Public on Aug 14, 2012 |
| Title |
10 mg/kg MPH tr1001 |
| Sample type |
RNA |
| |
|
| Source name |
mRNA of substantia nigra from 10mg/kg methylphenidate-treated mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: Swiss Webster
treatment: 10mg/kg MPH
tissue: substantia nigra
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA was isolated from SN in accordance with the protocol outlined in RNAqueous-Micro kit (Ambion, Austin, TX) according to manufacturer's recommendations.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix expression protocol from 100 nanograms total RNA (GeneChip 3' IVT Expression Kit, P/N 702646 Rev. 5).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 microgram of cRNA were hybridized for 16 hr at 45C on HT MG-430 PM array plate. Arrays were washed and stained in the Affymetrix GeneTitan.
|
| Scan protocol |
Arrays were scanned using the Affymetrix GeneTian
|
| Description |
Gene expression data from mRNA of substantia nigra from 10mg/kg methylphenidate-treated mouse
|
| Data processing |
The data were normalized using RMA method with arrays on the plate. Batch correction was done using ComBat.
|
| |
|
| Submission date |
Nov 10, 2011 |
| Last update date |
Aug 14, 2012 |
| Contact name |
Chunxu Qu |
| E-mail(s) |
[email protected]
|
| Phone |
9015952433
|
| Organization name |
St Jude Children's Research Institute
|
| Department |
Pathology
|
| Lab |
Mullighan
|
| Street address |
262 Danny Thomas Pl
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE33619 |
Methylphenidate exposure induces dopamine neuron loss and activation of microglia in the basal ganglia of mice |
|
