|
| Status |
Public on Nov 05, 2012 |
| Title |
Huh7 91 |
| Sample type |
RNA |
| |
|
| Source name |
Exosome
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Huh7
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The ExoQuicki kit (System Biosciences) was used to extract total RNA including microRNA from exosome fraction.
|
| Label |
Cy3
|
| Label protocol |
One-color microRNA labeling with Cy3 fluorescence were performed in accordance with the manufacturer’s procedure.
|
| |
|
| Hybridization protocol |
Hybridization were performed in accordance with the manufacturer’s procedure.
|
| Scan protocol |
The array was scanned with a laser scanner (G2565BA; Agilent).
|
| Data processing |
Feature Extraction 9.5 software (Agilent) was used to obtain the microarray raw data.
|
| |
|
| Submission date |
Nov 17, 2011 |
| Last update date |
Nov 05, 2012 |
| Contact name |
toshihito tanahashi |
| E-mail(s) |
[email protected]
|
| Organization name |
Tokushima University
|
| Street address |
Kuramoto-cho 3-18-15
|
| City |
Tokushima city |
| ZIP/Postal code |
7708503 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10656 |
| Series (1) |
| GSE33857 |
miRNA analysis is useful to diagnosis of liver disease, and evaluation of the grade of liver disease. |
|
