|
| Status |
Public on Jul 29, 2024 |
| Title |
Mixed_5555-mEGFP_DMSO [A3] |
| Sample type |
RNA |
| |
|
| Source name |
5555 melanoma cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 5555-mEGFP
tag: DMSO
|
| Treatment protocol |
MAF2-mCherry and 5555-mEGFP co-cultures were treated with DMSO (0.1%) or PLX4720 (1 mM) for 24 hours. mEGFP-positive fraction (5555-mEGFP) were FACS-sorted.
|
| Growth protocol |
Melanoma cells were maintained in DMEM (Invitrogen) with 10% fetal bovine serum/1% PenStrep (GIBCO).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using a Qiagen RNA extraction kit. The RNA was amplified using an Ambion Illumina TotalPrep RNA Amplification Kit.
|
| Label |
biotin
|
| Label protocol |
Standard Illumina biotin labelling protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
A3
|
| Data processing |
Raw data was transformed using variance-stabilizing transformation before quantile normalisation using the lumi package in R.
|
| |
|
| Submission date |
Jun 27, 2024 |
| Last update date |
Jul 29, 2024 |
| Contact name |
Probir Chakravarty |
| E-mail(s) |
[email protected]
|
| Organization name |
Francis Crick Institute
|
| Street address |
1 Midland Road
|
| City |
London |
| ZIP/Postal code |
NW1 1AT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE270933 |
Effect of PLX4720 on melanoma cells co-cultured with melanoma-associated fibroblasts |
|
