|
| Status |
Public on Sep 29, 2013 |
| Title |
LNCaP cells |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP
|
| Treatment protocol |
Cells were seeding in 6-cm petri dish (density 8x10^5 cells). After cells attach for 24h, 70 ug/ml BP were added for 3 or 24 hr
|
| Growth protocol |
LNCaP cells were growth in RPMI1640 medium with 10% FBS, 1% PS, at 37C/5 % CO2 incubator
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 45° for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 80μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 16 hours at 45° at 60rpm• Fluidics washing is FS450_0007
|
| Scan protocol |
Affymetrix GeneChip® Scanner 3000 7G, GCOS v1.4
|
| Description |
9SGST001
Gene expression data from LNCaP cells
|
| Data processing |
Raw intensity were background corrected and nomalized by Expression Console (RMA algorithsm). Gene level expression comparison were analyzed by R package.
|
| |
|
| Submission date |
Nov 22, 2011 |
| Last update date |
Sep 29, 2013 |
| Contact name |
Sheng-Chun Chiu |
| E-mail(s) |
[email protected]
|
| Organization name |
Buddhist TzuChi General Hospital
|
| Department |
Department of Medical Research
|
| Street address |
No. 707, Sec. 3, Chung Yang Rd
|
| City |
Hualien |
| ZIP/Postal code |
970 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE33883 |
Expression profile of LNCaP prostate cancer cell line treated with DMSO or BP |
|
