|
| Status |
Public on Jul 16, 2024 |
| Title |
H1299-KRAS-V12 |
| Sample type |
RNA |
| |
|
| Source name |
H1299 with mutant KRAS-V12 shRNA
|
| Organism |
Homo sapiens |
| Characteristics |
histology: Lung cancer cell line
subtype: Large cell carcinoma
|
| Growth protocol |
RPMI 5% BFS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit including DNase1 treatment
|
| Label |
biotin
|
| Label protocol |
biotin
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
|
| Data processing |
Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250
|
| |
|
| Submission date |
Jul 11, 2024 |
| Last update date |
Jul 16, 2024 |
| Contact name |
Noriaki Sunaga |
| E-mail(s) |
[email protected]
|
| Organization name |
Gunma University Graduate School of Medicine
|
| Department |
Department of Respiratory Medicine
|
| Street address |
3-39-15 Showa-machi
|
| City |
Maebashi |
| State/province |
Gunma |
| ZIP/Postal code |
371-8511 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE272045 |
Identification of mutant KRAS-related genes associated with malignant phenotypes in NSCLC |
|
