|
| Status |
Public on Jun 01, 2012 |
| Title |
ABI3:GR ABA induced 2 |
| Sample type |
RNA |
| |
|
| Source name |
whole seedling
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
developmental stage according to boyes et. al 2001: 1.02
genotype/variation: ABI3:GR
treatment: 4h treatment with ABA
|
| Treatment protocol |
Two weeks old seedlings of wildtype (WT, Col-0) and ABI3:GR transgenics were incubated in liquid MS media supplemented with either Abscisic acid (ABA;10 µM) or Dexamethasone (DEX; 30 µM) or both; an identical volume of ethanol used as solute for ABA and DEX was added in the mock treatment
|
| Growth protocol |
Seedlings were grown on MS-agar plates, supplemented with selective antibiotic, 16 h light/ 8h dark
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Plant Mini Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Cyanin 3 labeled cRNA was prepared from 100 ng total RNA using the Low Input Quick Amp Labeling Kit, One color (#5190-2305) of Agilent Technologies according to the manufacturers instructions
|
| |
|
| Hybridization protocol |
1.65 µg of Cy3 labelled cRNA was fragmented according to the manufacturer's instruction. 1.425 µg of fragmented cRNA (conc:15 ng/µl) was hybridized at 65°C, 10 rpm, 17 h in an Agilent hybridization oven
|
| Scan protocol |
Scanning was performed using an Agilent DNA Microarray Scanner (Model G2505C) and Scan Control Software (version A.8.3.1) of Agilent
|
| Description |
gene expression after 4h treatment with ABA
|
| Data processing |
Primary data analysis was performed using Agilent's Feature Extraction Software (version 10.7.3.1)
|
| |
|
| Submission date |
Nov 25, 2011 |
| Last update date |
Jun 01, 2012 |
| Contact name |
Gudrun Moenke |
| E-mail(s) |
[email protected]
|
| Organization name |
IPK
|
| Street address |
Corrensstr. 3
|
| City |
Gatersleben |
| ZIP/Postal code |
06466 |
| Country |
Germany |
| |
|
| Platform ID |
GPL12621 |
| Series (2) |
| GSE33951 |
Identification of target genes of ABI3 [Agilent 44k] |
| GSE34043 |
Identification of target genes of ABI3 |
|
