|
| Status |
Public on Nov 06, 2024 |
| Title |
Fibroblasts_T21_Sample5 |
| Sample type |
RNA |
| |
|
| Source name |
Cultured primary human fibroblasts
|
| Organism |
Homo sapiens |
| Characteristics |
genotype: T21
Sex: male
|
| Growth protocol |
Fibroblasts were maintained in DMEM high glucose with GlutaMAX, pyruvate, 10% FBS, and 100 U penicillin/ 100 µg streptomycin / ml. iPSCs were maintained on Vitronectin-XF-coated plates in StemFlex medium (A3349401, ThermoFisher Scientific). Neural progenitor cells were maintained on Matrigel-coated plates in STEMdiff Neural Progenitor Medium (05833, StemCell Technologies).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from 5 x 10^5 cells per line for each fibroblast, iPSC, and NPC line using NucleoSpin RNA XS Plus kits (Macherey-Nagel). RNA concentrations and quality were measured using a Nanodrop (ThermoFisher Scientific). RNA was converted to cDNA with GeneChip HT WT PLUS Reagent Kits (ThermoFisher Scientific).
|
| Label |
biotin
|
| Label protocol |
Samples were labeled with Biotin Allonamide Triphosphate using Human Clariom S HT arrays (Cat. No. 902970, Thermo Fisher Scientific) and the GeneTitan automated microarray system.
|
| |
|
| Hybridization protocol |
Arrays were washed and stained using the GeneTitan Hybridization, Wash, and Stain kit with streptavidin-phycoerythrin protocol according to the manufacturer's instructions.
|
| Scan protocol |
Arrays were scanned using the GeneTitan automated microarray system.
|
| Description |
Gene expression data from fibroblasts
|
| Data processing |
Data were processed with Partek Genomics Suite using robust multiarray average (RMA) background correction with quantile normalization and log2 transformation.
|
| |
|
| Submission date |
Aug 02, 2024 |
| Last update date |
Nov 06, 2024 |
| Contact name |
Laura L Baxter |
| Organization name |
NIH
|
| Department |
NHGRI
|
| Lab |
PGTS/CPHR
|
| Street address |
35A Convent Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL24324 |
| Series (1) |
| GSE273850 |
Expression data from human trisomy 21 and euploid fibroblasts, iPSCs, and neural progenitor cells |
|
