|
| Status |
Public on Jul 11, 2012 |
| Title |
Muscle KO Adult 0713 |
| Sample type |
RNA |
| |
|
| Source name |
Tibialis Anterior Muscle, Desmin Knockout, Adult
|
| Organism |
Mus musculus |
| Characteristics |
strain: Des(-/-)/129/SVE
genetic background: 129/SVE
tissue: tibialis anterior
age: 7-9 wk
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy Kit (Qaigen) extraction of total RNA was performed
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2-5 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip Mouse Genome 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS5) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 12, 2011 |
| Last update date |
Jul 11, 2012 |
| Contact name |
Gretchen Meyer |
| Organization name |
University of California San Diego
|
| Department |
Bioengineering
|
| Street address |
9500 Gilman Dr
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92122 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE34388 |
Transcriptional Alterations in Skeletal Muscle Following Desmin Deletion |
|
